| Gene | Slc27a3 |
| Chromosome | 3 |
| Deletion Start | 90,389,787 |
| Deletion End | 90,385,255 |
| Deletion Size | 4,533 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,389,062 | 90,389,083 | GAGGGATGTGCTAGACCCAAAG |
| TUP | 90,389,032 | 90,389,057 | CTTAAGCCGTCGGATTACAGGTCCCA |
| TUR | 90,388,992 | 90,389,011 | CTAGGGTGAGGGTGGCAATG |
| TDF | 90,385,501 | 90,385,524 | GCCACTACTGAGACCTTCAAACAG |
| TDP | 90,385,467 | 90,385,493 | TTAGGATGGCCAATGAGGGCTTTGACC |
| TDR | 90,385,443 | 90,385,463 | AGAGTGGGTCAGACAGTACAC |
PCR Primers
| Primer | Sequence |
| SU | GGAGGGTAGGCACCTCTTTG |
| SD | TTCACCCTTCCCTGAGTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17189A-B4 | VGB6 | C57BL/6NTac | |
| 17189A-B11 | VGB6 | C57BL/6NTac | |
| 17189A-D12 | VGB6 | C57BL/6NTac | |
| 17189A-G11 | VGB6 | C57BL/6NTac | |
| 17189A-H4 | VGB6 | C57BL/6NTac | |
| 17189A-F6 | VGB6 | C57BL/6NTac | |
