| Gene | Slc9a6 |
| Chromosome | X |
| Deletion Start | 56,638,383 |
| Deletion End | 56,609,863 |
| Deletion Size | 28,521 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,610,073 | 56,610,093 | AGCGCCAACCTGCTCATCTTC |
| TUP | 56,610,096 | 56,610,118 | CCTGCTGCTCACCCTCACCATTC |
| TUR | 56,610,160 | 56,610,177 | AGCAAGGCCGGTTTCGTG |
| TDF | 56,637,930 | 56,637,950 | AGAGCAACTCGCTGGTTGGAG |
| TDP | 56,637,951 | 56,637,978 | ATGAGAAGTGGGCATGAAGGGCTTACCA |
| TDR | 56,638,003 | 56,638,022 | CCCAGCCTAGCTTTCATCAC |
PCR Primers
| Primer | Sequence |
| SU | ACAGTGGATTAGATTCGATAAG |
| SD | CCTTTGAGGTTGGCAAAGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17196A-G3 | VGB6 | C57BL/6NTac | |
| 17196B-A7 | VGB6 | C57BL/6NTac | |
| 17196B-F7 | VGB6 | C57BL/6NTac | |
| 17196B-G4 | VGB6 | C57BL/6NTac | |
| 17196B-G12 | VGB6 | C57BL/6NTac | |
