| Gene | Tmem126a |
| Chromosome | 7 |
| Deletion Start | 90,455,509 |
| Deletion End | 90,450,683 |
| Deletion Size | 4,827 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,454,627 | 90,454,649 | AGGTATGCAACTCAAAGGTTGTC |
| TUP | 90,454,595 | 90,454,623 | CAGATTGATTCCACCCAGTCATGAGTGGG |
| TUR | 90,454,560 | 90,454,581 | TGATCCCGTTTCTGTTCTTCTC |
| TDF | 90,451,624 | 90,451,645 | GTGAAACCTGTACCACAACACG |
| TDP | 90,451,593 | 90,451,620 | TGCACTAGTTGGTTTGGTTATGGGTGGT |
| TDR | 90,451,562 | 90,451,585 | TGACGGGTATAGCCAAAAGGATAG |
PCR Primers
| Primer | Sequence |
| SU | CAGTCATGTCACCATGTCTG |
| SD | CTCTATGAGGAAACGTCCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17205A-B9 | VGB6 | C57BL/6NTac | |
| 17205A-F3 | VGB6 | C57BL/6NTac | |
