| Gene | Upk3b |
| Chromosome | 5 |
| Deletion Start | 136,044,095 |
| Deletion End | 136,038,805 |
| Deletion Size | 5,291 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,039,395 | 136,039,415 | GCCCAGGTGACCTTAAACTTC |
| TUP | 136,039,416 | 136,039,439 | TGAGCTTACTGCCTCCACCTTCCA |
| TUR | 136,039,463 | 136,039,482 | CACATGGACTGGCATGGTTG |
| TDF | 136,043,833 | 136,043,852 | AGGCTGACGGGTAAAGATTC |
| TDP | 136,043,858 | 136,043,884 | AAGGGACACAGCTAGAATGTGATGGCC |
| TDR | 136,043,886 | 136,043,907 | ACGACGTTACATCCCTAACCAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTGGATAAGGATGGATGG |
| SD | TACTGACTGCCCAACAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17233A-H10 | VGB6 | C57BL/6NTac | |
| 17233A-H4 | VGB6 | C57BL/6NTac | |
| 17233A-F3 | VGB6 | C57BL/6NTac | |
| 17233A-G10 | VGB6 | C57BL/6NTac | |
| 17233A-H11 | VGB6 | C57BL/6NTac | |
