| Gene | Zdhhc4 |
| Chromosome | 5 |
| Deletion Start | 143,326,212 |
| Deletion End | 143,316,465 |
| Deletion Size | 9,748 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,325,788 | 143,325,808 | CAGGTGTGAACTCAGGAGGAC |
| TUP | 143,325,759 | 143,325,786 | CAAGTGACCAGAAAGCTGAGCATCTAGC |
| TUR | 143,325,719 | 143,325,741 | AAGGGAAGACAGATGGGACAATG |
| TDF | 143,316,745 | 143,316,765 | TGGCTGGCTACTTGTGCTTTG |
| TDP | 143,316,721 | 143,316,743 | TCTGTACCTGGCTGCCACCAACC |
| TDR | 143,316,687 | 143,316,709 | GCCCAGTCGCCTTTATACCATTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGTGGTACTCACATATGCAC |
| SD | CTGGGAAAGAACAGACCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17237A-E12 | VGB6 | C57BL/6NTac | |
| 17237B-A9 | VGB6 | C57BL/6NTac | |
| 17237B-E12 | VGB6 | C57BL/6NTac | |
| 17237C-F3 | VGB6 | C57BL/6NTac | |
| 17237C-F2 | VGB6 | C57BL/6NTac | |
