17243 Gdf6 Status: ES cell colonies screened / QC no positives
| Gene | Gdf6 |
| Chromosome | 4 |
| Deletion Start | 9,860,330 |
| Deletion End | 9,844,481 |
| Deletion Size | 15,850 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,844,884 | 9,844,905 | GAGGACTGGGTAAGTGGCAAAG |
| TUP | 9,844,906 | 9,844,929 | AAAGCGCCCGACTCCTCTCCAGTT |
| TUR | 9,844,948 | 9,844,966 | TGCCAGAGCCTGGGTAGAG |
| TDF | 9,859,510 | 9,859,530 | ATTGCTACTGGACGCCAGGAC |
| TDP | 9,859,533 | 9,859,554 | TGGATCCTCAGGGACCAACCCA |
| TDR | 9,859,568 | 9,859,585 | CTGCCACACGTCGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | TCTTCCAGCTCTCACCTTGC |
| SD | AACCAATCTGAGATGCGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
