17248 Abcb1b Status: Cancelled
| Gene | Abcb1b |
| Chromosome | 5 |
| Deletion Start | 8,825,279 |
| Deletion End | 8,798,745 |
| Deletion Size | 26,535 |
| Genome Build | 38 |
|
| MGI ID | MGI:97568 |
| NCBI Gene ID | 0 |
| BAC ID | 382K13 |
| BAC Library | bMQ |
| Cassette | ZEN-Ub1 |
| Clones Available | No
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CTGTTGATCGCTCATGCCTTG |
| TUP | | | AGCCTTATGCTGCTTGGGTGCTCAC |
| TUR | | | TCACCTGTTATGCCACATTCTG |
| TDF | | | AAACCGGCCTAACATTGACTTCTC |
| TDP | | | TGTCTTGGTAGATCTTGAAGGGCCTCA |
| TDR | | | GTCTGTCCGCTCTTCACCTTC |
PCR Primers
| Primer | Sequence |
| SU | AGCATCAGGGCGGGGCTC |
| SD | AAGCTCCAGAAAATAGTCTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
