17251 Kcnq1 Status: Cancelled
| Gene | Kcnq1 |
| Chromosome | 7 |
| Deletion Start | 143,149,582 |
| Deletion End | 143,107,362 |
| Deletion Size | 42,221 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,107,802 | 143,107,826 | GCAAGGAAGGGCTAATCACAGAAAG |
| TUP | 143,107,827 | 143,107,852 | CAGAAACCAGACTGAGAGTCGGTCCC |
| TUR | 143,107,864 | 143,107,883 | CCACAAGCCACAGCAGAGTC |
| TDF | 143,148,938 | 143,148,960 | GACTTATCTTCCCAGTGCCCTTG |
| TDP | 143,148,962 | 143,148,986 | AATCAGAGCAGGCAGCAACTGTGCC |
| TDR | 143,149,018 | 143,149,036 | CCTGAACCTGTGCCCTGAG |
PCR Primers
| Primer | Sequence |
| SU | AGATAGCCTGACTTGGCTTG |
| SD | CTATTCTTCCTGCATCTTCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
