17263   Osbpl7     Status: ES cell colonies screened / QC positives

GeneOsbpl7
Chromosome11
Deletion Start97,068,104
Deletion End97,052,549
Deletion Size15,556
Genome Build38
MGI IDMGI:1918490
NCBI Gene ID0
BAC ID104N3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFGCTGCTTGTTCTGTCCCTCTG
TUPTACTTTGTGCTGGAGGACGGGATCCT
TURTGTCGAGTCGTGGCATAGTG
TDFTCCAGACCATGAGCGAAAC
TDPTCGGGTTCACTCAGTTTGCCCTGG
TDRAAGGCAGGGTCCGCTTCAG

PCR Primers

PrimerSequence
SUCTCGTGGGTCTCTCTCTTGC
SDCAGGGAAGATGCTGGAAGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17263A-A3VGB6C57BL/6NTac
17263A-B7VGB6C57BL/6NTac
17263A-H12VGB6C57BL/6NTac
17263A-G6VGB6C57BL/6NTac
17263A-E5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image