17278 Blvra Status: ES cell colonies screened / QC no positives
| Gene | Blvra |
| Chromosome | 2 |
| Deletion Start | 127,097,092 |
| Deletion End | 127,080,650 |
| Deletion Size | 16,443 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,081,026 | 127,081,046 | TGCACATGCTCTGGGAGATAG |
| TUP | 127,081,050 | 127,081,074 | AGGCATTAGGACCGCCTGGGCTCTT |
| TUR | 127,081,106 | 127,081,127 | ATCCGAACCCTGCTCATTCTAC |
| TDF | 127,096,440 | 127,096,462 | GTGGAAGCTGGAAGTTGATGTTG |
| TDP | 127,096,465 | 127,096,490 | TGCTATCGTCCTCCTTGACCAGTCTC |
| TDR | 127,096,509 | 127,096,531 | GCAAGGTCTGGACTCAGTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCAAGCTGTGTGTGTCTG |
| SD | TTATCATTTGCACCCTTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
