17282 1200009I06Rik Status: Cancelled
| Gene | 1200009I06Rik |
| Chromosome | 12 |
| Deletion Start | 111,430,882 |
| Deletion End | 111,422,082 |
| Deletion Size | 8,801 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,422,256 | 111,422,276 | ACCAGCCTGAGAACTTCAACC |
| TUP | 111,422,279 | 111,422,300 | CAAGCCCAAGGAGGACCCAGGC |
| TUR | 111,422,320 | 111,422,341 | CTCAAGGACCTGAACAGGAAAC |
| TDF | 111,430,300 | 111,430,320 | GCCTCTACCTCCCTAGCATTC |
| TDP | 111,430,321 | 111,430,342 | TGCCAGTTTCTGGAGCCTTCCC |
| TDR | 111,430,377 | 111,430,394 | GCGCACAGCGGAAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTTTGAGTCCAACTGGG |
| SD | CTCGGTCCTCCCATACATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
