17282 1200009I06Rik Status: Targeting vector QC completed
| Gene | 1200009I06Rik |
| Chromosome | 12 |
| Deletion Start | 112,660,293 |
| Deletion End | 112,669,093 |
| Deletion Size | 8,801 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,660,467 | 112,660,487 | ACCAGCCTGAGAACTTCAACC |
| TUP | 112,660,490 | 112,660,511 | CAAGCCCAAGGAGGACCCAGGC |
| TUR | 112,660,531 | 112,660,552 | CTCAAGGACCTGAACAGGAAAC |
| TDF | 112,668,511 | 112,668,531 | GCCTCTACCTCCCTAGCATTC |
| TDP | 112,668,532 | 112,668,553 | TGCCAGTTTCTGGAGCCTTCCC |
| TDR | 112,668,588 | 112,668,605 | GCGCACAGCGGAAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTTTGAGTCCAACTGGG |
| SD | CTCGGTCCTCCCATACATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
