17291 A230046K03Rik Status: Cancelled
| Gene | A230046K03Rik |
| Chromosome | 10 |
| Deletion Start | 83,573,878 |
| Deletion End | 83,544,045 |
| Deletion Size | 29,834 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,544,368 | 83,544,388 | CAGATGCTCAGCCTTCAGTTC |
| TUP | 83,544,389 | 83,544,408 | TAGAAGAGTCTGGGTTCCCG |
| TUR | 83,544,453 | 83,544,476 | GGTTCTAAGAACGTGAATCCAAAC |
| TDF | 83,572,965 | 83,572,985 | GGGTTGAACTAACGTGGGAAG |
| TDP | 83,572,988 | 83,573,014 | TTCCTGGAGGGACGTTGAACATTGAAC |
| TDR | 83,573,025 | 83,573,043 | TGCAATGCAAGAGCTGAAC |
PCR Primers
| Primer | Sequence |
| SU | ACAGAAAGGGAGCTGGTGAG |
| SD | ACTGAGTAACATGCAGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
