17293 Aass Status: Cancelled
| Gene | Aass |
| Chromosome | 6 |
| Deletion Start | 23,122,719 |
| Deletion End | 23,092,270 |
| Deletion Size | 30,450 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,122,582 | 23,122,601 | CTCTGGCTCCCAAGCACATC |
| TUP | 23,122,554 | 23,122,580 | AGGGCATCACCAAACTGGGCTACAAGG |
| TUR | 23,122,528 | 23,122,549 | CCGTCTGTTGGAAGGCTGTATC |
| TDF | 23,092,442 | 23,092,460 | AGCCACCGCCTCCAGTAAG |
| TDP | 23,092,417 | 23,092,440 | TGCCACCTCGGGATCGTTCCTCTT |
| TDR | 23,092,357 | 23,092,374 | TGCCAGCATCATCCACAC |
PCR Primers
| Primer | Sequence |
| SU | TCACAACGAGCATTTGATGAC |
| SD | CAACAGACAAAAATCCCAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
