17301 Ahcyl1 Status: Cancelled
| Gene | Ahcyl1 |
| Chromosome | 3 |
| Deletion Start | 107,696,274 |
| Deletion End | 107,665,105 |
| Deletion Size | 31,170 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,695,601 | 107,695,622 | AAATGGTAATCGCCGTGCAGTC |
| TUP | 107,695,570 | 107,695,598 | CCACCTGCTAGGAAGTATGTGAGAAAGGA |
| TUR | 107,695,529 | 107,695,549 | TGCATCCATGACAGTGTCTAC |
| TDF | 107,665,641 | 107,665,663 | CTGCCCTTACCACTTTCCTCTTC |
| TDP | 107,665,605 | 107,665,630 | ATGTTGCCAGCTTGCACTTACCATCA |
| TDR | 107,665,566 | 107,665,586 | TGCTTGGTCATCTGTCAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TGTTGCTTCTGGTTCTG |
| SD | GTACCATCCTGGCTCCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
