17303 Ak4 Status: Cancelled
| Gene | Ak4 |
| Chromosome | 4 |
| Deletion Start | 101,463,708 |
| Deletion End | 101,419,824 |
| Deletion Size | 43,885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,420,683 | 101,420,703 | AGATCGCCCCATCACTTAGTC |
| TUP | 101,420,704 | 101,420,726 | TTGCAAGTGTCTCCCTTCTCCCC |
| TUR | 101,420,750 | 101,420,771 | CACAGTAGCACGCGGATAACTC |
| TDF | 101,463,110 | 101,463,129 | GGTGATGGGAAGAGCACTGG |
| TDP | 101,463,130 | 101,463,157 | ACCAAGGCTAAGTGTAAAGGCTCGTTCC |
| TDR | 101,463,167 | 101,463,188 | ACCTCTCCACCACTTCGTCTAC |
PCR Primers
| Primer | Sequence |
| SU | CTTTCCACCGAGGTGTGTG |
| SD | GATGTCTGAGAGCCAGGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
