| Gene | Akr1a1 |
| Chromosome | 4 |
| Deletion Start | 116,645,584 |
| Deletion End | 116,636,503 |
| Deletion Size | 9,082 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,645,277 | 116,645,298 | GGAGCAGAGTCTTATGGTGGTG |
| TUP | 116,645,253 | 116,645,275 | TGGAGCCTCTTTGTTTCCAGGCC |
| TUR | 116,645,191 | 116,645,209 | CCGCTGTGCAGGAGATGTG |
| TDF | 116,636,655 | 116,636,674 | TTCAGGTGGATGGGAAGAGG |
| TDP | 116,636,630 | 116,636,652 | TCCCAGAGATGCTGGACACCCTC |
| TDR | 116,636,599 | 116,636,623 | AGGTCTCAGTATGGGTCATTAAAGG |
PCR Primers
| Primer | Sequence |
| SU | ATTTTAGCCTTGTGTGAAGGC |
| SD | TAGGCACTGACTAGGGCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17304A-H7 | VGB6 | C57BL/6NTac | |
| 17304A-H4 | VGB6 | C57BL/6NTac | |
| 17304A-H1 | VGB6 | C57BL/6NTac | |
| 17304A-G4 | VGB6 | C57BL/6NTac | |
