17305 Aldh3b1 Status: Cancelled
| Gene | Aldh3b1 |
| Chromosome | 19 |
| Deletion Start | 3,923,897 |
| Deletion End | 3,913,816 |
| Deletion Size | 10,082 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,923,664 | 3,923,687 | CAAGGGTGGTACAACTGGGATAAG |
| TUP | 3,923,639 | 3,923,662 | AGCACGGTTTAGGCACATCCTCCA |
| TUR | 3,923,617 | 3,923,637 | CTGGGCTTTCAGCTCCTCTTC |
| TDF | 3,914,115 | 3,914,136 | CCCTCAGTTGGCCCTTAAAGAC |
| TDP | 3,914,088 | 3,914,112 | AAAGACCCATGTTCTGTGCTGCCCA |
| TDR | 3,914,019 | 3,914,038 | AGGCACGCTGGTTGGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTCCTGTAAGTCCATCTG |
| SD | CTGTAGCTTGGAGTCAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
