17307 Anapc4 Status: Cancelled
| Gene | Anapc4 |
| Chromosome | 5 |
| Deletion Start | 52,866,620 |
| Deletion End | 52,834,267 |
| Deletion Size | 32,354 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,834,818 | 52,834,838 | GCCTCACAGAATTGCAGACAG |
| TUP | 52,834,841 | 52,834,865 | TGCGTGTGGACGTTGTCAGTTAGCA |
| TUR | 52,834,910 | 52,834,932 | TCCTCCACAGAAAGATGGGTATC |
| TDF | 52,865,899 | 52,865,918 | ACGAGCAGGGCAGCATTATC |
| TDP | 52,865,920 | 52,865,943 | CCACACGCACCATGCACTTTGAGA |
| TDR | 52,865,978 | 52,865,996 | GCCGTTCCCAGCAACATAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTGTGCCACTCACTTCC |
| SD | AAGTGTACCGCTCCTGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 115 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
