17308 Ankrd24 Status: Cancelled
| Gene | Ankrd24 |
| Chromosome | 10 |
| Deletion Start | 81,647,225 |
| Deletion End | 81,629,716 |
| Deletion Size | 17,510 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,629,855 | 81,629,873 | TGCAGCAGGTCAGCTACTC |
| TUP | 81,629,877 | 81,629,899 | CAGGTCAGCTGCTCCAGCAGGTC |
| TUR | 81,629,926 | 81,629,947 | GTGAACCTACGTGGGCAATCAG |
| TDF | 81,646,822 | 81,646,843 | GGCTTTATACCGCACCCATCTC |
| TDP | 81,646,845 | 81,646,871 | TGTATGCCATTCAGGTGAGTCCTCTGC |
| TDR | 81,646,903 | 81,646,922 | CGAGGCCCAGGTAGTGTCAG |
PCR Primers
| Primer | Sequence |
| SU | GTTCTGTGTGGGAGAGAGGC |
| SD | CTGCCCTTACCCAGTGTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
