| Gene | Atg2a |
| Chromosome | 19 |
| Deletion Start | 6,261,859 |
| Deletion End | 6,241,799 |
| Deletion Size | 20,061 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,241,859 | 6,241,879 | CAGCACTACTTGGGTCACTTC |
| TUP | 6,241,880 | 6,241,902 | TTTCAGGAGCACCTCAGCCTGGA |
| TUR | 6,241,958 | 6,241,976 | GCCTCCTCACCCAGGTTTC |
| TDF | 6,261,450 | 6,261,468 | CGGAAGCTACGCAGAGGTC |
| TDP | 6,261,470 | 6,261,494 | ACAACCTGCTGACCTCCGGGAAGGC |
| TDR | 6,261,530 | 6,261,549 | CAAGTGGGTCTAGGGTGGTC |
PCR Primers
| Primer | Sequence |
| SU | CATTTCATCTCGCAAAGAGC |
| SD | GACCCACATGGCATCTATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17313A-A2 | VGB6 | C57BL/6NTac | |
| 17313A-A4 | VGB6 | C57BL/6NTac | |
| 17313A-C5 | VGB6 | C57BL/6NTac | |
| 17313A-G11 | VGB6 | C57BL/6NTac | |
| 17313A-E9 | VGB6 | C57BL/6NTac | |
