| Gene | Bbs5 |
| Chromosome | 2 |
| Deletion Start | 69,667,254 |
| Deletion End | 69,647,287 |
| Deletion Size | 19,968 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,647,409 | 69,647,428 | CAGCGGCCTATCTCAGTGTG |
| TUP | 69,647,429 | 69,647,451 | CGCCCATCAAGGCTGTGACCCTC |
| TUR | 69,647,524 | 69,647,543 | GAGGCCCGTAGTGTGCAATG |
| TDF | 69,666,309 | 69,666,330 | CAGCCTTCTCACAGCTCCAAAG |
| TDP | 69,666,331 | 69,666,352 | CCTGCTGTCTCCTCTCAGGCCA |
| TDR | 69,666,373 | 69,666,391 | CCGCTCTGAGCAGTTCATC |
PCR Primers
| Primer | Sequence |
| SU | CAGCAGGAAAGCTGAAATGG |
| SD | TAAATCCAACATGATACAATCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 135 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17316A-A1 | VGB6 | C57BL/6NTac | |
| 17316A-C12 | VGB6 | C57BL/6NTac | |
| 17316A-F4 | VGB6 | C57BL/6NTac | |
| 17316A-H12 | VGB6 | C57BL/6NTac | |
