| Gene | Cc2d2a |
| Chromosome | 5 |
| Deletion Start | 43,700,074 |
| Deletion End | 43,668,671 |
| Deletion Size | 31,404 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,669,464 | 43,669,486 | CAGATAAGGGAAGCCACCTACAG |
| TUP | 43,669,487 | 43,669,509 | TGGAGACTGTGCCCACTCAGCAG |
| TUR | 43,669,524 | 43,669,547 | CCAAGTGGGAGTAAGGGTTTGAAG |
| TDF | 43,699,954 | 43,699,976 | GCGAGATGCTGAACAAGAGAAAG |
| TDP | 43,699,979 | 43,700,008 | CGAACACTGCTGAAGACCATCATAAAGGTC |
| TDR | 43,700,023 | 43,700,044 | CCTCTGGAACTCCCGTAATGAC |
PCR Primers
| Primer | Sequence |
| SU | GCTGGGTGAAAGCAGAGAAG |
| SD | TGTATTCTCAAGGGTTATACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17322B-B7 | VGB6 | C57BL/6NTac | |
| 17322B-D3 | VGB6 | C57BL/6NTac | |
| 17322B-E5 | VGB6 | C57BL/6NTac | |
| 17322C-H11 | VGB6 | C57BL/6NTac | |
| 17322C-E1 | VGB6 | C57BL/6NTac | |
| 17322C-A7 | VGB6 | C57BL/6NTac | |
