17336 Ctcf Status: Cancelled
| Gene | Ctcf |
| Chromosome | 8 |
| Deletion Start | 105,681,638 |
| Deletion End | 105,663,766 |
| Deletion Size | 17,873 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,663,984 | 105,664,006 | GACTGAAGTCATGGAGGGTACAG |
| TUP | 105,664,010 | 105,664,031 | CTCCGGAAGCAGAGGCTGCAGT |
| TUR | 105,664,036 | 105,664,060 | CCTGCAAGGTTATGATCTGGGTATC |
| TDF | 105,681,300 | 105,681,317 | TGCTGACTTGGGCATCAC |
| TDP | 105,681,318 | 105,681,341 | TGCTGAGGCTTTCTTGTTGCTGCA |
| TDR | 105,681,364 | 105,681,382 | GCGAGCACAGGCATTGTTC |
PCR Primers
| Primer | Sequence |
| SU | ATTCATTCACTAAGGTGGGTT |
| SD | CATGCCATCCTACTGGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
