17338   D9Ertd402e     Status: ES cell colonies screened / QC no positives

GeneD9Ertd402e
Chromosome9
Deletion Start122,835,064
Deletion End122,808,811
Deletion Size26,254
Genome Build38
MGI IDMGI:1196217
NCBI Gene ID382117
BAC ID118G15
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 9

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF122,809,215122,809,237CAGCTTGGATAGGTGGGAGTTTG
TUP122,809,247122,809,276AAATCATGTAAACGGGAAGGCTAAGGGAGA
TUR122,809,291122,809,314TCATCTCTTCTGGAGAACCCTCAG
TDF122,834,891122,834,914TCTGCGTCTCACATTTCTATTCTG
TDP122,834,921122,834,946AAGATGGAGACCTGTGCATCCCGTGG
TDR122,834,955122,834,978TGTTACTTCATGGCTTCTCCTTTC

PCR Primers

PrimerSequence
SUCTAAACCACATCCTCACACG
SDCTGGCTACAAAGTGTGTGTGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR100 bp++-
TDFTDR88 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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