| Gene | Gabrp |
| Chromosome | 11 |
| Deletion Start | 33,573,367 |
| Deletion End | 33,552,492 |
| Deletion Size | 20,876 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,573,052 | 33,573,074 | AAGGCCCTTGGTGAAAGAAATAG |
| TUP | 33,573,015 | 33,573,041 | TCAGTGGCTAAGGGTGCTTACTGTGTA |
| TUR | 33,572,982 | 33,573,001 | GGACACTGGAACCTGGACTC |
| TDF | 33,552,818 | 33,552,838 | CAGCGAAGGACTCGGAAGAAG |
| TDP | 33,552,783 | 33,552,812 | ATCACCAACATCATCAACAGCTCCATCTCC |
| TDR | 33,552,747 | 33,552,768 | TTCAATGCTGGCAAAGCTAATC |
PCR Primers
| Primer | Sequence |
| SU | TGTGCTGTGGACTGGCTATG |
| SD | AGGCAAGGTAACCACCAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17365A-A9 | VGB6 | C57BL/6NTac | |
| 17365A-B6 | VGB6 | C57BL/6NTac | |
| 17365A-B8 | VGB6 | C57BL/6NTac | |
| 17365A-C5 | VGB6 | C57BL/6NTac | |
| 17365A-E11 | VGB6 | C57BL/6NTac | |
