| Gene | Hmha1 |
| Chromosome | 10 |
| Deletion Start | 80,031,078 |
| Deletion End | 80,019,732 |
| Deletion Size | 11,347 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,020,405 | 80,020,425 | TCCTCTGGCTCTGATGCTTTC |
| TUP | 80,020,426 | 80,020,447 | CGTGGCTCCCTGCTGCTCAGAG |
| TUR | 80,020,488 | 80,020,508 | GTCAGTAGTCACCCAGCTTTC |
| TDF | 80,030,799 | 80,030,817 | GGACGCCAGTGCTCTACAC |
| TDP | 80,030,818 | 80,030,841 | CGCCTCAGTTTCCTGGAGCAGACC |
| TDR | 80,030,872 | 80,030,894 | AGCTGTTCCTCACTGCCACTATG |
PCR Primers
| Primer | Sequence |
| SU | CTGCCCTGAGCATCCAACTC |
| SD | GTCTGTCCATGTCCAGCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17385A-D11 | VGB6 | C57BL/6NTac | |
| 17385A-E2 | VGB6 | C57BL/6NTac | |
| 17385A-F4 | VGB6 | C57BL/6NTac | |
| 17385A-F7 | VGB6 | C57BL/6NTac | |
| 17385A-G5 | VGB6 | C57BL/6NTac | |
