| Gene | Irf1 |
| Chromosome | 11 |
| Deletion Start | 53,776,546 |
| Deletion End | 53,771,292 |
| Deletion Size | 5,255 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,771,496 | 53,771,516 | GAACGTGCAAGGTCTGACTTC |
| TUP | 53,771,517 | 53,771,540 | TGCCTCCTGCTTCTTCCTTCCTGA |
| TUR | 53,771,553 | 53,771,575 | GACTGTACCACTAGGAGGGTGAG |
| TDF | 53,776,343 | 53,776,365 | TCACGGAGATGAAGAATATGGAC |
| TDP | 53,776,368 | 53,776,393 | CATCATGTGGATGGACAGCCTGCTGG |
| TDR | 53,776,395 | 53,776,413 | CGGCAGCCTCACAGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | ATCCATCCAGACTTGCTTGG |
| SD | GGGCCAGTGCTATGCTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17394A-D10 | VGB6 | C57BL/6NTac | |
| 17394A-A7 | VGB6 | C57BL/6NTac | |
| 17394A-A10 | VGB6 | C57BL/6NTac | |
| 17394A-D5 | VGB6 | C57BL/6NTac | |
| 17394A-D9 | VGB6 | C57BL/6NTac | |
| 17394A-B4 | VGB6 | C57BL/6NTac | |
