17402 Nprl3 Status: Cancelled
| Gene | Nprl3 |
| Chromosome | 11 |
| Deletion Start | 32,267,537 |
| Deletion End | 32,232,956 |
| Deletion Size | 34,582 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,267,082 | 32,267,100 | CTGGTCCATGCCCATTGTG |
| TUP | 32,267,046 | 32,267,075 | TGCTCACCATCTTGATAATCATCCCTCCTG |
| TUR | 32,267,016 | 32,267,037 | TTTGGTGGGTACACTTTCAGAG |
| TDF | 32,233,370 | 32,233,390 | GTGCCGTGGAAGAACAGAAAG |
| TDP | 32,233,345 | 32,233,369 | AAGCGCACTCCTCCCTTCAGTAGGG |
| TDR | 32,233,308 | 32,233,325 | GCATGGCTGTGCAGACTC |
PCR Primers
| Primer | Sequence |
| SU | GACTTTAAACTGCAGACAGC |
| SD | CATTGATGGCACGCGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
