| Gene | Mras |
| Chromosome | 9 |
| Deletion Start | 99,411,582 |
| Deletion End | 99,388,321 |
| Deletion Size | 23,262 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,411,183 | 99,411,203 | CGAAAGTGGACACCGAACAAG |
| TUP | 99,411,157 | 99,411,181 | AGGCCTTGCTGGACTCCTGACAGCT |
| TUR | 99,411,126 | 99,411,148 | CAAGCAAGCAACACCTGACATAG |
| TDF | 99,388,653 | 99,388,674 | TGCACTAAGAGATGCCACACAG |
| TDP | 99,388,632 | 99,388,652 | CCTGGTCTCCTTGGGTGGCCA |
| TDR | 99,388,599 | 99,388,618 | GGTTGGAGGGACAGCTTGAG |
PCR Primers
| Primer | Sequence |
| SU | AGCCCAGAGCCCTCTTACTC |
| SD | CAGAGGTCTGCCAAAGACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17410A-A7 | VGB6 | C57BL/6NTac | |
| 17410A-G12 | VGB6 | C57BL/6NTac | |
