| Gene | Nat2 |
| Chromosome | 8 |
| Deletion Start | 67,502,213 |
| Deletion End | 67,501,243 |
| Deletion Size | 971 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,501,591 | 67,501,615 | GACTACATTGTTGATGCTGGGTTTG |
| TUP | 67,501,618 | 67,501,642 | CGTTCCTACCAGATGTGGGAGCCTC |
| TUR | 67,501,658 | 67,501,678 | GCACCTGAGGCTGATCCTTTC |
| TDF | 67,501,877 | 67,501,901 | CAGCGTCTGTGTTTACTAGCAAATC |
| TDP | 67,501,905 | 67,501,928 | TTGTTCCCTGCAGACCCCAGAAGG |
| TDR | 67,501,930 | 67,501,950 | GGAGCCAACCAAACAATGAAC |
PCR Primers
| Primer | Sequence |
| SU | TTGTTATTTGTCCTTGTCTGCC |
| SD | AATTGGTGTTTGTTCCCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17422A-A11 | VGB6 | C57BL/6NTac | |
| 17422A-B2 | VGB6 | C57BL/6NTac | |
| 17422A-H9 | VGB6 | C57BL/6NTac | |
| 17422A-D11 | VGB6 | C57BL/6NTac | |
| 17422A-D1 | VGB6 | C57BL/6NTac | |
