17426 Sirt1 Status: Cancelled
| Gene | Sirt1 |
| Chromosome | 10 |
| Deletion Start | 63,338,967 |
| Deletion End | 63,320,600 |
| Deletion Size | 18,368 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,338,300 | 63,338,319 | CTCGCAGTTTGGCGTTAGAG |
| TUP | 63,338,268 | 63,338,292 | TTCCCTCCCTCGGTTTGGTTTCCCT |
| TUR | 63,338,206 | 63,338,228 | CCAAAGTCGCAAAGCAAAGTGTG |
| TDF | 63,321,110 | 63,321,133 | TGGAAGTATGTTTCTGGTCATTGG |
| TDP | 63,321,082 | 63,321,109 | TGCAAACAAGGGAGCTGTTAGACTTGAA |
| TDR | 63,321,024 | 63,321,046 | TGGCTTACTCTTGAACTCAACTC |
PCR Primers
| Primer | Sequence |
| SU | CATCGCAAACTTGAACCACC |
| SD | GCAGAACAGAAATTGAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
