17429 Nfxl1 Status: Cancelled
| Gene | Nfxl1 |
| Chromosome | 5 |
| Deletion Start | 72,559,263 |
| Deletion End | 72,529,016 |
| Deletion Size | 30,248 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,558,894 | 72,558,914 | AACAGCTCCCGGAGTTTAGTC |
| TUP | 72,558,870 | 72,558,893 | TCAGGGAACCGTCTCCATTCAGGC |
| TUR | 72,558,837 | 72,558,859 | AAAATGAGGATGCAAGCGATGAG |
| TDF | 72,529,422 | 72,529,441 | CAGTACCCTGTGGCCGAGAG |
| TDP | 72,529,393 | 72,529,418 | ACCACAAGGCCACCTAAGTGCAAAGA |
| TDR | 72,529,350 | 72,529,371 | TCAGATGTTGGGCTGTGGAATG |
PCR Primers
| Primer | Sequence |
| SU | GAGGCTGAGGAGAATTTCC |
| SD | CCTTCCAGTCTTGGGTAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
