17430 Nkrf Status: Cancelled
| Gene | Nkrf |
| Chromosome | X |
| Deletion Start | 36,891,736 |
| Deletion End | 36,888,463 |
| Deletion Size | 3,274 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,891,591 | 36,891,615 | CCTTGTTTCTGTAAAGTGGGATGTG |
| TUP | 36,891,558 | 36,891,585 | TCTGGCTTCTTGAAATGTGTCACATGGC |
| TUR | 36,891,525 | 36,891,546 | GCATAGAATCCAACGCTACCAG |
| TDF | 36,888,751 | 36,888,774 | CCACTCAGATTTGACCTTCTCCAC |
| TDP | 36,888,718 | 36,888,745 | TGACCAATGATGAACGGAAGCAAATCCA |
| TDR | 36,888,679 | 36,888,702 | TGAGACTTACTCTTCAGGCCATAC |
PCR Primers
| Primer | Sequence |
| SU | CAGCCCCTGTGTTTGTATATG |
| SD | CAACTGGCTTCTCACAAAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
