17443 Pcbd1 Status: Cancelled
| Gene | Pcbd1 |
| Chromosome | 10 |
| Deletion Start | 61,094,003 |
| Deletion End | 61,089,417 |
| Deletion Size | 4,587 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,089,994 | 61,090,013 | GCCCACACTAACAAGCTGAC |
| TUP | 61,090,016 | 61,090,045 | AGCTTCCCTAAGCAAATAAGGCCCTTACCC |
| TUR | 61,090,055 | 61,090,075 | GGCTGACAATGCTCTGAACTC |
| TDF | 61,093,815 | 61,093,835 | CACCTTGAGCACCCATGAATG |
| TDP | 61,093,836 | 61,093,858 | TGCCGGTCTTTCGGAACGGGATA |
| TDR | 61,093,872 | 61,093,893 | CACGGCGACTTGTTCGATGAAG |
PCR Primers
| Primer | Sequence |
| SU | TGTGACATTTATCTGGGTGGC |
| SD | TCTAGAAGCTGAGCCCATCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
