17449   Pfkl     Status: ES cell colonies screened / QC positives

GenePfkl
Chromosome10
Deletion Start78,009,680
Deletion End77,988,150
Deletion Size21,531
Genome Build38
MGI IDMGI:97547
NCBI Gene ID18641
BAC ID86J16
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF78,009,03678,009,054AACAGGGCAAGCACCACAG
TUP78,009,00678,009,028AGTTGAGGGTGCACAGCCTCTTC
TUR78,008,96378,008,981GATCCTCCCAAGCCCAGAC
TDF77,988,63977,988,658CTGAGTGGAGAAGGGAGGAC
TDP77,988,61477,988,636TGGGAGGCCCAGAACTTTCCAGG
TDR77,988,56177,988,581CATTGAACGCATGGACAAGAC

PCR Primers

PrimerSequence
SUTCAGTCAGGCTCAGGTTGTG
SDTGCACCTGAGTCCTGACAAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR92 bp++-
TDFTDR98 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17449A-A10VGB6C57BL/6NTac
17449A-H1VGB6C57BL/6NTac
17449A-H10VGB6C57BL/6NTac
17449A-H11VGB6C57BL/6NTac