Gene | Pfkl |
Chromosome | 10 |
Deletion Start | 78,009,680 |
Deletion End | 77,988,150 |
Deletion Size | 21,531 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 78,009,036 | 78,009,054 | AACAGGGCAAGCACCACAG |
TUP | 78,009,006 | 78,009,028 | AGTTGAGGGTGCACAGCCTCTTC |
TUR | 78,008,963 | 78,008,981 | GATCCTCCCAAGCCCAGAC |
TDF | 77,988,639 | 77,988,658 | CTGAGTGGAGAAGGGAGGAC |
TDP | 77,988,614 | 77,988,636 | TGGGAGGCCCAGAACTTTCCAGG |
TDR | 77,988,561 | 77,988,581 | CATTGAACGCATGGACAAGAC |
PCR Primers
Primer | Sequence |
SU | TCAGTCAGGCTCAGGTTGTG |
SD | TGCACCTGAGTCCTGACAAC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 92 bp | + | + | - |
TDF | TDR | 98 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
17449A-A10 | VGB6 | C57BL/6NTac | |
17449A-H1 | VGB6 | C57BL/6NTac | |
17449A-H10 | VGB6 | C57BL/6NTac | |
17449A-H11 | VGB6 | C57BL/6NTac | |