17452 Pik3cb Status: Cancelled
| Gene | Pik3cb |
| Chromosome | 9 |
| Deletion Start | 99,105,680 |
| Deletion End | 99,073,582 |
| Deletion Size | 32,099 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,104,774 | 99,104,792 | GCCACGACTGGCTTAGAAC |
| TUP | 99,104,746 | 99,104,773 | TCATAGCTGAATACCTCCCTTGGCCTTC |
| TUR | 99,104,724 | 99,104,745 | CCCTCACCCACATTAGCACTTG |
| TDF | 99,074,555 | 99,074,577 | GCATTTCTTGTGCTGGCAAGTAG |
| TDP | 99,074,531 | 99,074,554 | TTCCTGTATGCCATGTCAGGGTGC |
| TDR | 99,074,491 | 99,074,513 | TGAGCAGCTCTGATTACCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | AACTGGTCGTAGATGGGCAG |
| SD | GTCTTTACTTTCCATGTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
