17460 Ppm1f Status: Cancelled
| Gene | Ppm1f |
| Chromosome | 16 |
| Deletion Start | 16,924,119 |
| Deletion End | 16,903,392 |
| Deletion Size | 20,728 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,903,397 | 16,903,414 | TGGAGCCGCACAGAACAG |
| TUP | 16,903,415 | 16,903,438 | CAGCCAGATGGCCTGTGACAGTGA |
| TUR | 16,903,459 | 16,903,480 | GCTGGGAAGTCTTGGAGGAATG |
| TDF | 16,923,290 | 16,923,310 | ACCTGCCTCTTCCTGTAACTC |
| TDP | 16,923,315 | 16,923,340 | TCCATCTACTCCATCTACACCTGCCC |
| TDR | 16,923,350 | 16,923,370 | GGAGCCAACAAAGACCTCAAG |
PCR Primers
| Primer | Sequence |
| SU | GGAAACATGCATTCCCTCTG |
| SD | TACAGACCAAACCCTCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
