| Gene | Rab19 |
| Chromosome | 6 |
| Deletion Start | 39,389,830 |
| Deletion End | 39,383,923 |
| Deletion Size | 5,908 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,384,051 | 39,384,070 | GTCCCAGCAGAACACCATTG |
| TUP | 39,384,074 | 39,384,095 | TGGACTTCACCGTGCGCTCCCT |
| TUR | 39,384,099 | 39,384,121 | CCTTCACTTTCTTGCCGTCTATC |
| TDF | 39,389,666 | 39,389,684 | TTTGTGCTCATGGCGAAGG |
| TDP | 39,389,685 | 39,389,709 | AGTTAATTGCCCGCAACAGCCTGCA |
| TDR | 39,389,736 | 39,389,756 | GGCTGGAATCCTGGGAAAGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGTTGTGGTGGGTTGACTG |
| SD | CTGGCTGTGACTGTTGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17477A-A8 | VGB6 | C57BL/6NTac | |
| 17477A-C3 | VGB6 | C57BL/6NTac | |
| 17477A-D10 | VGB6 | C57BL/6NTac | |
| 17477A-H6 | VGB6 | C57BL/6NTac | |
| 17477A-F11 | VGB6 | C57BL/6NTac | |
| 17477A-F12 | VGB6 | C57BL/6NTac | |
