17480 Rapsn Status: Cancelled
| Gene | Rapsn |
| Chromosome | 2 |
| Deletion Start | 91,045,558 |
| Deletion End | 91,035,800 |
| Deletion Size | 9,759 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,036,173 | 91,036,192 | GGCTGGGTATCGTCAGAGTC |
| TUP | 91,036,196 | 91,036,219 | CCTGTGGCCTTTGACCTCGGTGTC |
| TUR | 91,036,229 | 91,036,248 | AAGATCCCTGGATGCCTGTG |
| TDF | 91,044,722 | 91,044,739 | GGGTGGGCAGGCTATTTC |
| TDP | 91,044,741 | 91,044,762 | CCCAGGACCCACAGCAAAGCAA |
| TDR | 91,044,783 | 91,044,802 | CGAGAGGCAGGGCTAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TCCAAAGAGCATAACCTGCC |
| SD | TTCAGCAGCAGGGATCTTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
