17484 Rftn2 Status: Cancelled
| Gene | Rftn2 |
| Chromosome | 1 |
| Deletion Start | 55,226,256 |
| Deletion End | 55,194,247 |
| Deletion Size | 32,010 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,226,165 | 55,226,186 | GACCGCAAGTGGAAACAAAGAC |
| TUP | 55,226,134 | 55,226,159 | TTGCTTATGAATACGCCCTGCTGGAC |
| TUR | 55,226,042 | 55,226,061 | TCCACAGGTCCACACTTCAG |
| TDF | 55,194,669 | 55,194,690 | GGCCACTGCTGAACCTACTTAG |
| TDP | 55,194,647 | 55,194,668 | TAAGCCTCGTGGATCGGACGCC |
| TDR | 55,194,577 | 55,194,596 | CTGGGAAAGCCACGGTTCTC |
PCR Primers
| Primer | Sequence |
| SU | TGTAAATTGACGTGGCATGG |
| SD | CGACCGGTGTGTGTAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 145 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
