| Gene | Rmnd5a |
| Chromosome | 6 |
| Deletion Start | 71,440,136 |
| Deletion End | 71,413,254 |
| Deletion Size | 26,883 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,439,259 | 71,439,279 | GCAGGTGAGATTAAGGCAGTG |
| TUP | 71,439,232 | 71,439,257 | TAGCCCTGCCTCTCCTCTCCTTTCCT |
| TUR | 71,439,175 | 71,439,195 | CTTTACCAGGGAGGAGCAGTC |
| TDF | 71,413,585 | 71,413,604 | TTGGCCTGAGTGTGCAGAAC |
| TDP | 71,413,558 | 71,413,581 | ACTCAGCTGGAGTCCACGGCTGGT |
| TDR | 71,413,514 | 71,413,534 | TTGTCCCACCTCTATGCTACC |
PCR Primers
| Primer | Sequence |
| SU | CCCGTCTTGCCTTCGCCT |
| SD | AAAGAGGTGTTGCAGCATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17487A-F7 | VGB6 | C57BL/6NTac | |
| 17487A-G12 | VGB6 | C57BL/6NTac | |
| 17487A-F9 | VGB6 | C57BL/6NTac | |
| 17487A-G8 | VGB6 | C57BL/6NTac | |
| 17487A-A10 | VGB6 | C57BL/6NTac | |
