17493 Sarnp Status: ES cell colonies screened / QC no positives
| Gene | Sarnp |
| Chromosome | 10 |
| Deletion Start | 128,848,785 |
| Deletion End | 128,821,939 |
| Deletion Size | 26,847 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,822,634 | 128,822,656 | TCGAGTTTTCTCAGGAGGTTCTC |
| TUP | 128,822,658 | 128,822,680 | ATGACCTGCCCTCCCTCCCCTGA |
| TUR | 128,822,699 | 128,822,722 | CAGCTATCTACCCTACACGTTCTC |
| TDF | 128,848,238 | 128,848,258 | GAATGCAGAAGAGGGCTGAAC |
| TDP | 128,848,269 | 128,848,295 | ACCTGTAAGCCTGGAGAGTAAGAAGGC |
| TDR | 128,848,311 | 128,848,333 | TCCAAACAGGTAGAAGCACTCAC |
PCR Primers
| Primer | Sequence |
| SU | CCTGTGGTCTCCAGGAAGTG |
| SD | ATAACTTCAGGACGTTCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
