17495   Scyl3     Status: ES cell colonies screened / QC positives

GeneScyl3
Chromosome1
Deletion Start163,941,214
Deletion End163,933,532
Deletion Size7,683
Genome Build38
MGI IDMGI:1921385
NCBI Gene ID240880
BAC ID226H3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF163,933,995163,934,018CCCTTAAAGTCACTGCTGCATTTG
TUP163,934,031163,934,060AGTGCAGTCAGTTTGCTATTTGGTTCCATG
TUR163,934,093163,934,117GACTGAGACATATCATCAGCTCAAG
TDF163,940,526163,940,547GCTGCCACATTCAGTCCTTTAG
TDP163,940,548163,940,573TGGCCTGTCTCATTCTGCTGACACCT
TDR163,940,601163,940,621AACACCAGACACTGCACATAC

PCR Primers

PrimerSequence
SUAACTGCACGTGACTGTTTGG
SDAGACTAAGGGAACCATCTAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR123 bp++-
TDFTDR96 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17495A-E7VGB6C57BL/6NTac
17495A-H11VGB6C57BL/6NTac
17495A-H2VGB6C57BL/6NTac
17495A-E9VGB6C57BL/6NTac
17495A-D1VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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