| Gene | Setd1a |
| Chromosome | 7 |
| Deletion Start | 127,799,580 |
| Deletion End | 127,778,131 |
| Deletion Size | 21,450 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,778,234 | 127,778,254 | CCCAGAAGGTGTACCGCTATG |
| TUP | 127,778,256 | 127,778,281 | CGGAGTCCACTTCAGTGTCAGTGTGA |
| TUR | 127,778,294 | 127,778,318 | TGGAGGTCCAAATAAGTCAAGAAAG |
| TDF | 127,798,746 | 127,798,766 | CCATCTGCCATCTGCTCTCAC |
| TDP | 127,798,767 | 127,798,788 | CAGCTGCCCAGTAGGTGCCTGT |
| TDR | 127,798,833 | 127,798,853 | GCAGCTTCCGAAATGACAGTG |
PCR Primers
| Primer | Sequence |
| SU | CTCTGGTTCGGAGGATCG |
| SD | GAACAGATGAGGCAGGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17501A-D5 | VGB6 | C57BL/6NTac | |
| 17501A-D7 | VGB6 | C57BL/6NTac | |
| 17501A-F4 | VGB6 | C57BL/6NTac | |
| 17501A-G6 | VGB6 | C57BL/6NTac | |
