| Gene | Shq1 |
| Chromosome | 6 |
| Deletion Start | 100,671,058 |
| Deletion End | 100,648,166 |
| Deletion Size | 22,893 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,670,885 | 100,670,903 | CTGCGTGCCGCTTTCATTC |
| TUP | 100,670,858 | 100,670,881 | AGCCCACGCTCGCGATTTACTCCG |
| TUR | 100,670,783 | 100,670,803 | AGACTGCGAGCCTGGAAGAAG |
| TDF | 100,648,511 | 100,648,530 | GAGGGCTGTGGTCAGTGTAG |
| TDP | 100,648,485 | 100,648,508 | TAAGAGCTCAGGGCAGGACAGCTC |
| TDR | 100,648,408 | 100,648,429 | CTCCCGTAAAGCAAACGGTAAC |
PCR Primers
| Primer | Sequence |
| SU | CGTTAGTCCGTAAACGTCC |
| SD | GGACCCTACTGATAGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17506B-B7 | VGB6 | C57BL/6NTac | |
| 17506B-C6 | VGB6 | C57BL/6NTac | |
| 17506B-G7 | VGB6 | C57BL/6NTac | |
| 17506C-A5 | VGB6 | C57BL/6NTac | |
| 17506C-D9 | VGB6 | C57BL/6NTac | |
