| Gene | Spg20 |
| Chromosome | 3 |
| Deletion Start | 55,135,701 |
| Deletion End | 55,116,989 |
| Deletion Size | 18,713 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,117,276 | 55,117,295 | GGGCCTTGCCACTTCTCTAC |
| TUP | 55,117,300 | 55,117,322 | TGACCTTCAGGATGTGCCCAAGC |
| TUR | 55,117,329 | 55,117,349 | CGTCTTTAGGCGGGAACTCTG |
| TDF | 55,134,918 | 55,134,938 | CCAGGGCGTCTGTGTACTTTG |
| TDP | 55,134,943 | 55,134,968 | TCTACAGACAGCAAGATGGCCAGTGA |
| TDR | 55,134,995 | 55,135,014 | GCAGAGACACTGTGAGGTTC |
PCR Primers
| Primer | Sequence |
| SU | GGTTAGCACTCAAGAAGAGG |
| SD | GAAATCACGTGCACAGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17511A-A4 | VGB6 | C57BL/6NTac | |
| 17511A-A5 | VGB6 | C57BL/6NTac | |
| 17511A-A11 | VGB6 | C57BL/6NTac | |
| 17511A-H11 | VGB6 | C57BL/6NTac | |
| 17511A-H3 | VGB6 | C57BL/6NTac | |
