| Gene | Spopl |
| Chromosome | 2 |
| Deletion Start | 23,545,518 |
| Deletion End | 23,511,241 |
| Deletion Size | 34,278 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,545,436 | 23,545,460 | GCTGGTGTTACACACAGGTACATAC |
| TUP | 23,545,398 | 23,545,426 | TGCATTTGCTGCTTGTCACATGCATTTTC |
| TUR | 23,545,338 | 23,545,362 | TCTAACGAGAGTGATAAAGCCATAC |
| TDF | 23,511,647 | 23,511,664 | TAGCCGTCAGTGGAGAGC |
| TDP | 23,511,624 | 23,511,646 | CGTGGGAATGACGACACGCGTCT |
| TDR | 23,511,584 | 23,511,603 | TTTCGGGCAAACCTGAACAG |
PCR Primers
| Primer | Sequence |
| SU | AGGAGATGAGCCAGACATCG |
| SD | GCAACGGGCTCTTTAGTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17513B-F4 | VGB6 | C57BL/6NTac | |
| 17513C-A5 | VGB6 | C57BL/6NTac | |
| 17513C-F6 | VGB6 | C57BL/6NTac | |
| 17513C-F11 | VGB6 | C57BL/6NTac | |
| 17513C-G7 | VGB6 | C57BL/6NTac | |
