| Gene | Stk17b |
| Chromosome | 1 |
| Deletion Start | 53,776,635 |
| Deletion End | 53,757,475 |
| Deletion Size | 19,161 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,776,615 | 53,776,635 | TCTCGGAGGAGATTCGATTGC |
| TUP | 53,776,585 | 53,776,614 | CGAAGTGTCTCAGGCTTGCTAACTACAACC |
| TUR | 53,776,503 | 53,776,527 | CAAAGTTTCCTTACCTCCCAAGTTC |
| TDF | 53,758,249 | 53,758,270 | GTGCTACTGAGTGGTGAGGAAG |
| TDP | 53,758,217 | 53,758,246 | TGGAATGCAGGCTAAAGACATGAGTCATGA |
| TDR | 53,758,174 | 53,758,198 | GACAGGGTTGAAACTAGATAGACAG |
PCR Primers
| Primer | Sequence |
| SU | TACCTCCTTCCACCTGCATC |
| SD | TACCCTTGACAGCATTTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 133 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17517A-B11 | VGB6 | C57BL/6NTac | |
| 17517A-C4 | VGB6 | C57BL/6NTac | |
| 17517A-E2 | VGB6 | C57BL/6NTac | |
| 17517A-B4 | VGB6 | C57BL/6NTac | |
