17525 Rdh11 Status: Cancelled
| Gene | Rdh11 |
| Chromosome | 12 |
| Deletion Start | 79,191,787 |
| Deletion End | 79,176,059 |
| Deletion Size | 15,729 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,190,986 | 79,191,008 | GTGACGGTGGACCAGTAATAAAC |
| TUP | 79,190,962 | 79,190,984 | CCACCTTCTTTCTGGGCGTGGCC |
| TUR | 79,190,934 | 79,190,954 | GCACAGCCTCAGCTCCAATAG |
| TDF | 79,176,834 | 79,176,851 | TAAGTGGGCCAGGCAGTC |
| TDP | 79,176,806 | 79,176,831 | TACTCAACCACTGAGGTAGCTCCCGA |
| TDR | 79,176,774 | 79,176,795 | GCAGAAGGCATTGTTACAGGAG |
PCR Primers
| Primer | Sequence |
| SU | ACCAACTGTCCGACTAACGG |
| SD | AGATGTTGGGACCACAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
