Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

17545 Upf3b Status: ES cell colonies screened / QC one positive

Gene	Upf3b
Chromosome	X
Deletion Start	37,110,228
Deletion End	37,092,508
Deletion Size	17,721
Genome Build	38

MGI ID	MGI:1915384
NCBI Gene ID	68134
BAC ID	27L19
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	37,109,966	37,109,989	CCGGGTAACTCTTCTGTTTCTAAC
TUP	37,109,935	37,109,961	TGGAAATTGCTTCGCACTGTCTCACGC
TUR	37,109,910	37,109,933	ACCTGCTAATCCCATTTCCACCAG
TDF	37,093,004	37,093,022	CCAGGGAGGCTGAGGTAAG
TDP	37,092,968	37,092,996	TAAGGTCAAGGCTGCCTGAGCAATTTAGT
TDR	37,092,913	37,092,933	TGCTCCACTGAGCCATATTCC

PCR Primers

Primer	Sequence
SU	TTGCATGCTGGGAGTTGTAG
SD	TGCTCCAAGCCAGGATTAAG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	80 bp	+	+	-
TDF	TDR	110 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
17545A-H9	VGB6	C57BL/6NTac